Immunohistochemical Detection of Avian Pneumovirus in Formalin-Fixed Tissues
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چکیده
منابع مشابه
Immunohistochemical detection of P-glycoprotein in formalin-fixed and paraffin-embedded normal and neoplastic canine tissues.
Expression of P-glycoprotein, a phylogenetically conserved integral plasma membrane protein, is implicated as one of the most important factors contributing to tumor cell multidrug resistance. Formalin-fixed, paraffin-embedded normal and neoplastic canine tissues were studied using an avidin-biotin complex technique employing three murine monoclonal antibodies (C494, C219, JSB-1) to different e...
متن کاملImmunohistochemical detection of DNA topoisomerase I in formalin fixed, paraffin wax embedded normal tissues and in ovarian carcinomas.
AIMS To determine, by in situ immunohistochemistry, whether ovarian carcinomas have increased expression of DNA topoisomerase I. METHODS Paraffin wax blocks obtained from 15 samples of normal human tissues and from 14 cases of ovarian cancer were cut on to glass slides and immunohistochemically stained for topoisomerase I. The primary antibody was a mouse monoclonal that recognises topoisomer...
متن کاملQuantitative Comparison of Immunohistochemical Staining Intensity in Tissues Fixed in Formalin and Histochoice
Formaldehyde fixatives have traditionally been used to preserve tissues as they impart excellent morphological preservation. Formaldehyde fixes tissue by cross linking, a process which can reduce the antigenicity of tissue and weakens the intensity of immunohistochemical stains. Preliminary studies have shown that Histochoice tissue fixative offers equal or greater staining intensity than neutr...
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Jorge W. López4, Charles V. Trimarchi2, Alexander I. Wandeler3, Yvonne van Gessel1 1From Diagnostic Laboratory, Cornell College of Veterinary Medicine Ithaca, NY 2Rabies Laboratory, N.Y. State Department of Health, Albany, NY 3Rabies Unit, Animal Disease Research Institute, Nepean, ONT 4Present address Pan American Foot-and-Mouth Disease Center (PAHO/WHO) PO Box 589, 20001-970 Rio de Janeiro, B...
متن کاملAn enhanced antigen-retrieval protocol for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Formalin is the most commonly used fixative for light microscopy because of its preservation of -morphological details. A major adverse effect of formalin fixation is formation of cross-linkages between epitopes (amino acid residues) and unrelated proteins by formaldehyde groups. The great majority of monoclonal and polyclonal antibodies used for immunohistochemical (IHC) staining of formalin-f...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2001
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063870101300103